Synergistic Effects of Vitis vinifera L. and Centella asiatica against CCl4-Induced Liver Injury in Mice

Liver injury can be acute or chronic, resulting from a variety of factors, including viral hepatitis, drug overdose, idiosyncratic reaction, toxins, while the progression pathogenesis in liver rises due to involvement numerous cytokines and growth factor mediators. Thus, identification more effective biomarker-based active phytochemicals isolated medicinal plants is promising strategy protect against CCl4-induced injury. Vitis vinifera L. (VE) Centella asiatica (CE) are well-known that possess anti-inflammatory antioxidant properties. However, synergism between two has not previously been studied. Here, we investigated synergistic effects V. leaf, C. extract combination (VCEC) Acute was induced by single intraperitoneal administration CCl4 (1 mL/kg). VCEC administered orally for three consecutive days at various concentrations (100 200 mg/kg) prior injection. The extent protective were evaluated biochemical analysis histopathological studies. Oxidative stress measuring malondialdehyde (MDA) glutathione (GSH) levels Western blotting. treatment significantly reduced serum transaminase (AST ALT), tumor necrosis factor-α (TNF-α), reactive oxygen species (ROS). CCl4- apoptosis inhibited reducing cleaved caspase-3 Bcl2-associated X protein (Bax). VCEC-treated mice restored cytochrome P450 2E1, nuclear erythroid 2-related 2 (Nrf2), heme oxygenase-1 (HO-1) expression CCl4-treated mice. In addition, downregulated overexpression proinflammatory hepatic kappa B (NF-κB) CCl4-mediated apoptosis. Collectively, exhibited through its antioxidant, anti-inflammatory, antiapoptotic ability oxidative stress, inflammation, Therefore, appears as potential therapeutic agent